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DNA

Part:BBa_K1732001:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-03)


J23100-CDcel-GlucCO-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1614
    Illegal BamHI site found at 1065
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 805


Design Notes

The sequence is codon optimized for E.coli.

The plasmid was used to produce Gaussia princeps luciferase proteins that would express luminescence when reacted with an optimal concentration of 1 uM coelenterazine. The CDcel domain allowed for the proteins to be secreted into the media (extracellular) which was both the expected and observed location.

CDcel Gaussia Sequence.jpg

Source

Provided by the Bruchez lab at Carnegie Mellon University.

References